School of Forest, Fisheries, and Geomatics Sciences, University of Florida
Contact
sneupane@ufl.edu
Research Summary
My research interests are in studying important aspects of host-vector-pathogen relationship in both pathology and entomology using both computational and molecular biology tools. I have been involved in the projects with specific aims that provide data and information to address the critical gap in fundamental knowledge of the role of the arthropods, nematodes and pathogens in plant health. The innovative aspects of the projects are that it integrates multidisciplinary cutting-edge tools available to conduct studies for agricultural benefit. The experiments are expected to provide fundamental knowledge related to the role of key genes in host-vector-pathogen systems using next generation sequencing data.
As a postdoctoral research associate at Kirsten Pelz-Stelinski’s lab, I was involved in projects aiming pest and pathogen management by changing an endosymbiont of insect vectors (Diaphorina citri, Lucilia sericata and Drosophila melanogaster). I used both Pacbio and illumina sequencing data to reconstruct the complete genome of the Wolbachia endosymbiont of the Asian Citrus Psyllid for the first time.
Currently, I am a postdoctoral research associate at Hulcr’s Lab. My work will be focused on studying the transcriptomes of the mycangia of ambrosia beetles to understand the beetle fungus symbiosis.
Short Bio & Education
- 2022-Present: Postdoctoral Research Associate: Hulcr’s lab, School of Forest, Fisheries, and Geomatics Sciences, University of Florida, USA
- 2019-2022: Postdoctoral Research Associate: Pelz-Stelinski’s lab, Citrus Research and Education Center, University of Florida, USA
- 2015-2019: PhD. Molecular Biology. South Dakota State University, USA
- 2012-2015: M.S. Thesis and Assistant Research Fellow. Nepal Academy of Science and Technology (NAST), Nepal
- 2010-2012: M.S. Biotechnology. Tribhuvan University, Nepal
- 2006-2009: B.S. Microbiology. Tribhuvan University, Nepal
- Andersen, E.J., S. Ali, R.N. Reese, Y. Yen, S. Neupane et al., 2016. Diversity and Evolution of Disease Resistance Genes in Barley (Hordeum vulgare L.). Evolutionary Bioinformatics Online 12:99.
- Neupane, S., J. Sijapati, T. Bhattarai, and S.J. Shrestha, 2017. Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers. African Journal of Biotechnology 16 (16):895-907.
- Nepal, M.P., E.J. Andersen, S. Neupane, and B.V. Benson, 2017. Comparative Genomics of Non-TNL Disease Resistance Genes from Six Plant Species. Genes 8 (10):249.
- Neupane, S., Q. Ma, F.M. Mathew, A.J. Varenhorst, E.J. Andersen et al., 2018. Evolutionary Divergence of TNL Disease-Resistant Proteins in Soybean (Glycine max) and Common Bean (Phaseolus vulgaris). Biochemical Genetics. 10.1007/s10528-018-9851-z
- Neupane, S., E.J. Andersen, A. Neupane, and M.P. Nepal, 2018. Genome-Wide Identification of NBS-Encoding Resistance Genes in Sunflower (Helianthus annuus L.). Genes 9 (8). 10.3390/genes9080384
- Neupane, S., S.E. Schweitzer, A. Neupane, E.J. Andersen, A. Fennell et al., 2019. Identification and Characterization of Mitogen-Activated Protein Kinase (MAPK) Genes in Sunflower (Helianthus annuus L.). Plants 8 (2):28.
- Neupane, S., A.J. Varenhorst, and M.P. Nepal, 2019. Transcriptome profiling of induced susceptibility effects on soybean–soybean aphid (Hemiptera: Aphididae) interaction. BMC Research Notes 12 (1):325. 10.1186/s13104-019-4372-3
- Neupane, S., F.M. Mathew, A.J. Varenhorst, and M.P. Nepal, 2019. Transcriptome profiling of interaction effects of soybean cyst nematodes and soybean aphids on soybean. Scientific Data 6 (1):133. 10.1038/s41597-019-0140-4
- Neupane, S., J.M. Purintun, F.M. Mathew, A.J. Varenhorst, and M.P. Nepal, 2019. Molecular Basis of Soybean Resistance to Soybean Aphids and Soybean Cyst Nematodes. Plants 8 (10):374.
- Chen, X.D., S. Neupane, T.A. Gill, H. Gossett, K.S. Pelz-Stelinski et al., 2021. Comparative transcriptome analysis of thiamethoxam susceptible and resistant Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), using RNA-sequencing. Insect Science 28 (6):1708-1720. https://doi.org/10.1111/1744-7917.12901
- Chen, X.D., S. Neupane, H. Gossett, K.S. Pelz-Stelinski, and L.L. Stelinski, 2021. Insecticide rotation scheme restores insecticide susceptibility in thiamethoxam-resistant field populations of Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), in Florida. Pest Management Science 77 (1):464-473. https://doi.org/10.1002/ps.6039
- Neupane, S., S.I. Bonilla, A.M. Manalo, and K.S. Pelz-Stelinski, 2020 Near-Complete Genome Sequences of Wolbachia Strain Isolated from Diaphorina citri Kuwayama. Microbiology resource announcements 9 (35):e00560-00520. 10.1128/MRA.00560-20
- Neupane, S., S.I. Bonilla, A.M. Manalo, and K.S. Pelz-Stelinski, 2022 Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing. Scientific reports 12 (1):125. 10.1038/s41598-021-03184-0
soybean cyst nematode interaction studies in soybean plants during PhD at SDSU