Adam,
Thank you in advance for your help with Latasha. Here is a summary of what we will do with Latasha on her first visit:
If she has beetles, we will identify them, and grind them up to extract symbionts. Here is how. We will plate them and wait a week.
If she doesn’t have beetles, or only species that are not easy to tell a story about, we will use our fungi.
– Raffaelea subfusca from Craig
– Ambrosiella hartigii from Martin Sigut
– Fusarium sp. from Martin Sigut
– Raffaelea lauricola from Marc
– Diplodia from oaks as a free-living control (or some better species?)
If these fungi are in decently clean cultures, we can subculture them directly into a competitive assay. If they need some cleanup, we will first subculture them alone.
Competitive assay: We will record single-direction reactions: each field has a record of the reactions of the fungus in the upper row. We also need reactions of each to itself, and a growth rate without a competitor. Can we do two replicates of each assay?
| R sub | R lau | A har | F sp | Dipl | |
|---|---|---|---|---|---|
| R sub | same-growth decrease? | R lau reaction | A har reaction | F sp reaction | Dipl reaction |
| R lau | R sub reaction | same-growth decrease? | A har reaction | etc | etc |
| A har | |||||
| F sp | |||||
| Dipl | |||||
| no competitor | R sub growth | etc |
Better ideas welcome!
How should we score it? How about Strong inhibition, medium, weak, no effect? Or estimated percentage of growth decrease compared to the no-competitor control??
Thank you!
