Adam,
Whenever you have a moment, will you be able to try to remove one of the unnecessary bench parts that are preventing people from sitting down with a microscope?
Thanks!

Adam,
We bought the Ampure DNA purification system. Would you be able to put it together, explore how it works, and give us training?
It might be worth taking a PCR product that is no longer needed but had a strong band (something that we already sequenced) and runningon a gel 10 5ul of the original, 2) 5ul Ampure cleaned-up version, and 3) 5ul MoBio cleaned version.
The sooner the better (within two or three weeks?), because I am imagining that Craig and possibly Tyler Dreaden might want to use it for the next MiSeq library.
For individual Sanger sequencing submission, we will still be using ExoSap. This is only for large volumes that have to be really clean, like whole library submissions.
Thank you!
J

Adam, in “your box” in my office is a sample of wood. Could you please take a photo of the bark side (female butts visible – please be careful, they are dead, old and fragile!) and of the internal winding frass-filled tunnels? I would like to show you in person first… This is for a book, you will be included as the photographer.

Adam,

Can you please recommend vendors and models for these items, based on the lab needs:

– second, bigger and better -80 freezer

– additional thermal cycler

– Adobe Creative suite, multi-user or floating license

– up-to-date Geneious, multi-user or floating license

– large-ish fridge for fungal culture storage (we are expecting quite a few in the Spring)

Please go for quality rather than low price, but try to keep the total within about $30,000. It is for the IFAS Equipment grant (Jason and I applying together), due January 15, so our budget needs to be done early in January to be vetted by Tim White.

Thank you!

Adam,

Could you please dissect the branches in my office (in blue tupperware box)? Please separate beetles that are already out on the paper (they will have fungi in the mycangium) and those that you pull out from the wood – these will include mature specimens, but also hopefully immature adults, pupae and larvae. We want all these developmental stages preserved in ethanol.

Please do it sooner rather than later – they are coming out now, and we need to process it before all the immatures develop. ‘Have you done this before? Please let me know if you have any questions!

Adam,

After your return, but by December 17, will you be able to help me with this:

1) Check out the species in the list below: do we have  >5 of each in our collection? Please send a list and numbers.

2) Of those that we have enough, could you let me see the ones marked as “have Jiri confirm ID”

3) and then point-mount ONE, provide them with labels, and pack them up in a small box for me.

Ambrosiodmus devexulus
Ambrosiophilus mogia Hulcr and Cognato sp. n.
Ambrosiophilus restrictus (Schedl 1939)
Arixyleborus simplicaudus Hulcr and Cognato sp. n.
Beaverium perplexus (Schedl 1969b)
Cyclorhipidion bituberculatum (Eggers 1923)
Cyclorhipidion multipunctatum (Browne 1980)
Cyclorhipidion spurlinum Hulcr and Cognato sp. n.
Debus spinicornis (Schedl 1975b)
Euwallacea andreae Hulcr and Cognato sp. n.
Euwallacea funereus (Lea 1910)
Hypothenemus distinctus
Hypothenemus gossypii
Hypothenemus miles
Hypothenemus pubescens
Hypothenemus setosus
Microperus intermedius (Eggers 1923) – have Jiri confirm ID before mounting
Microperus parvus (Lea 1893) – have Jiri confirm ID before mounting
Microperus pometianus (Schedl 1939a) – have Jiri confirm ID before mounting
Planiculus immersus (Schedl 1972b)
Planiculus rodmanculus Hulcr and Cognato sp. n.
Theoborus ricini
Trischidias atomus
Trischidias minutissimus
Trischidias striatus
Truncaudum longior (Eggers 1923)
Wallacellus (Euwallacea) striatulus (Browne 1980)
Xyleborus (Ewallacea) declivispinatus (Schedl 1969b) restor. Comb.
Xyleborus bispinatus Eichhoff (1868) from New Guinea
Xylosandrus russulus (Schedl 1942c)

Adam, apparently this is now supposed to work, so i can start using it, to both ask for help with things, and also to keep a record of it.

Could you please buy a new kettle with much lower wattage for the student office? Please put it on 60460004-182-3300-CRRNT-Hulcr-FY14-15? The current one blew the circuit again. Please let me know if none of the cheap ones seems compatible with our neolithic circuits.

Thank you

Adam,

Also a secondary priority, but when you get he chance:

1) In the “Adam” box in my office, there is a box with samples that need to be returned to Japan. It has instructions.

2) In the sample freezer door, there is a mini ziploc bag with a couple of vials. They need to be incorporated into the collection and database. Here are the data:

③-1     Xylosandrus discolor

③-2     Xylosandrus discolor

⑤-2     Xylosandrus crassiusculus

⑤-3     Xylosandrus crassiusculus

⑫         Diuncus corpulentus

Japan, Iriomote Is.

2014

Kajimura coll.

Adam,

This is NOT a priority, but when you have a minute, could you get together these two samples:

– vial 3323 (10 Xyleborus glabratus).

– vial 7459 (1 male of X. glabratus)

Make sure these are the only specimens in there (if not, separate them) and send them to Shaun Bennett in New Zealand. Here is his email:

—————————–

Hello Jiri

Thank you, and Paul, so much for your help.

Some specimens for our two laboratory reference collections will be greatly received.

Courier is best – our FedEx account number is 2373 4453 7

How do you plant to send the specimens – dry or wet? Our importation requirements mean the specimens are best sent in a small amount of ethanol, or ethanol soaked cotton wool/tissue – makes coming through our own Ministry inspectors straight forward.

Courier address is:

Shaun J. Bennett
Plant Health & Environment Laboratory

Ministry for Primary Industries

231 Morrin Road, St Johns, Auckland 1072

New Zealand

Let me know if you need any more info.  Appreciate if you could let me know when they are on their way.

Cheers

Shaun

—————————

After that, please record the samples as loaned to Shaun Bennett.

Thank you!

Adam,
Please stop by when you have a second. I’d like to ask you what you think about the list of insecticides for wood borers that we talked about some time ago. You mentioned that you have a preliminary list – can we take a look at it together? If it looks like a viable miniproject, I would like to assign it to a student in ENY, but would like to know first what you have found out so far. I am meeting with an Entomology Dept. specialist to discuss this at the end of next week, and would like to see something before that. Thanks!
Cheers,
J