Materials
- grinding tool
- scalpel
- hood time
- strong forceps
- vials
- ethanol in petri dish
- vial rack
Procedure
Scrape piece of 1 gallery in the hood, scrape into
a. 1 ml of 1X PBS AUTOCLAVED phosphate buffer saline solution in tube
3. mortar & pestle
4. plate directly, refrigeration decreases growing success
5. Plate three different dilutions, refer to the appropriate protocol.
