- Place a drop of 70-95% ethanol on a microscope slide.
- Immerse the fungal material in the drop of alcohol.
- Add one or at most two drops of the lactophenol blue stain before the alcohol dries out.
- Holding the coverslip between forefinger and thumb, touch one edge of the drop of alcohol/stain with the coverslip edge, and lower gently to avoid air bubbles.
- Ring the edge of the coverslip with nail polish to prevent desiccation This way, the mount will last for days to months.
