Using multimode detector (plate reader) Beckman Coulter DTX 880 to measure absorbance at 630nm with a 20×20 detector grid, using sterile 24-well Costar plate with a lid against contamination, 1 ml of media per well. Measured on the first day and on fourth day, comparing (cellulose media minus negative control) versus (positive control minus negative control).
Vortex chunk of fungus mycelium, take 0.1 ml of supernatant, add to 0.9 ml of media.