Protocol
Cellulose Degradation
Isolate possible cellulose degraders on CMC (5 g/L & salts, false positives possible) or MCC (4g/L & salts). Use CMC media for Congo Red test – grow isolate in the plate center for a week, overlay with Congo Red, wash with water, wash with salts (3M NaCl), check for clearing. When isolating cellulose degrading bacteria from wood, it’s good to add antifungal antibiotics, otherwise Trichoderma takes over.
