Bacteria and fungi (in yeast-like form, larger than yeast cells) are packed together with non-soluble oils. Can use tween, but that sometimes decrease germination. Spread manually on plates.Surface-sterilize (or rather wash) beetles either three times in sterile water for 1 min, or 30s in White’s solution and then in 1 min in sterile water (Six & Bentz, 2007).